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Import/export projects

Import/export projects

This tutorial shows how to import and export single-particle and tomography projects. nextPYP supports importing Sessions, CLI projects, or Relion 4.0 projects. We will demonstrate how to import/export data to and from Relion (the other options work similarly).

1. Import single-particle projects

  • Create or navigate to an existing single-particle project, click Import Data and select Single-particle (from Star)

  • Go to the Import parameters tab:

    • Set the location of the Relion refinement star file by clicking on the icon and browsing to the corresponding directory

    • Set the location of the Relion motioncorr star file by clicking on the icon and browsing to the corresponding directory

    • Set the location of the Relion project path by clicking on the icon and browsing to the corresponding directory

    • Go to the Raw data tab:

    • Set the Location of the raw data by clicking on the icon and browsing to the corresponding directory

    • Click on the Microscope parameters tab

    • Set Pixel size (A)

    • Set Acceleration voltage (kV)

    • (optional) Set parameters in other tabs

  • Click Save and the new block will appear on the project page

  • Click Run followed by Start Run for 1 block to launch the import process

  • Click inside the block to inspect the results

2. Import tomography projects

  • Create or navigate to an existing tomography project, click Import Data and select Tomography (from Star)

  • Go to the Import parameters tab:

    • Set the location of the Relion refinement star file by clicking on the icon and browsing to the corresponding directory

    • Set the location of the Relion tomogram star file by clicking on the icon and browsing to the corresponding directory

    • Set the location of the Relion project path by clicking on the icon and browsing to the corresponding directory

    • Go to the Raw data tab:

    • Set the Location of the raw data by clicking on the icon and browsing to the corresponding directory

    • Click on the Microscope parameters tab

    • Set Pixel size (A)

    • Set Acceleration voltage (kV)

    • Set Tilt-axis angle (degrees)

    • (optional) Set parameters in other tabs

  • Click Save and the new block will appear on the project page

  • Click Run followed by Start Run for 1 block to launch the import process

  • Click inside the block to inspect the results

3. Export single-particle/tomography projects

  • Choose an existing Particle refinement block, click on the menu icon and select the Edit option

  • Go to the Export tab:

    • Check Export metadata

    • Set the location of the Input parfile you want to export by clicking on the icon and browsing to the corresponding directory

  • Click Save

  • Click Run followed by Start Run for 1 block to launch the export process

Once the job ends, the results will appear in the specified folder and will be ready to import into other packages. For a tomography project example, you can use Relion’s Import Coordinates procedure

See also

previous

Tomography session

next

User guide