Import/export projects
Contents
Import/export projects¶
This tutorial shows how to import and export single-particle and tomography projects. nextPYP
supports importing Sessions, CLI projects, or Relion 4.0 projects. We will demonstrate how to import/export data to and from Relion (the other options work similarly).
1. Import single-particle projects¶
Create or navigate to an existing single-particle project, click Import Data and select Single-particle (from Star)
Go to the Import parameters tab:
Set the location of the
Relion refinement star file
by clicking on the icon and browsing to the corresponding directorySet the location of the
Relion motioncorr star file
by clicking on the icon and browsing to the corresponding directorySet the location of the
Relion project path
by clicking on the icon and browsing to the corresponding directory
Go to the Raw data tab:
Set the
Location
of the raw data by clicking on the icon and browsing to the corresponding directoryClick on the Microscope parameters tab
Set
Pixel size (A)
Set
Acceleration voltage (kV)
(optional) Set parameters in other tabs
Click Save and the new block will appear on the project page
Click Run followed by Start Run for 1 block to launch the import process
Click inside the block to inspect the results
2. Import tomography projects¶
Create or navigate to an existing tomography project, click Import Data and select Tomography (from Star)
Go to the Import parameters tab:
Set the location of the
Relion refinement star file
by clicking on the icon and browsing to the corresponding directorySet the location of the
Relion tomogram star file
by clicking on the icon and browsing to the corresponding directorySet the location of the
Relion project path
by clicking on the icon and browsing to the corresponding directory
Go to the Raw data tab:
Set the
Location
of the raw data by clicking on the icon and browsing to the corresponding directoryClick on the Microscope parameters tab
Set
Pixel size (A)
Set
Acceleration voltage (kV)
Set
Tilt-axis angle (degrees)
(optional) Set parameters in other tabs
Click Save and the new block will appear on the project page
Click Run followed by Start Run for 1 block to launch the import process
Click inside the block to inspect the results
3. Export single-particle/tomography projects¶
Choose an existing Particle refinement block, click on the menu icon and select the Edit option
Go to the Export tab:
Check
Export metadata
Set the location of the
Input parfile
you want to export by clicking on the icon and browsing to the corresponding directory
Click Save
Click Run followed by Start Run for 1 block to launch the export process
Once the job ends, the results will appear in the specified folder and will be ready to import into other packages. For a tomography project example, you can use Relion’s Import Coordinates procedure