Single-particle session

This tutorial shows how to process micrographs on-the-fly during data collection. nextPYP implements streaming using three parallel tracks:

  • File transfer from the microscope computer to a permanent storage location

  • Data pre-processing consisting of frame alignment, CTF estimation and particle picking

  • 2D classification to monitor particle quality

Each track is displayed in a separate row and has its own Status indicator and Controls to Start, Restart, Clear, or Stop the session and to display the Logs

Step 1: Create a new session

Step 2: Launch the streaming session

Step 3 (optional): Adjust data processing parameters

Step 4: Copy or delete a session

Step 5 (optional): Filter micrographs and export metadata