nextPYP: a scalable platform for cryo-EM/ET image analysis

nextPYP is a package for single-particle cryo-EM/ET image analysis developed and maintained by the Bartesaghi Lab.

By combining established tools in the field with methods developed in-house, nextPYP produces state-of-the-art results while being user-friendly and scalable to large datasets.

Main features

  • Ease-of-use: user-friendly, fully-featured web-based GUI with intuitive block-based architecture

  • End-to-end: convert raw cryo-EM/ET data into high-resolution structures using a single application

  • Particle picking suite: cellular pattern mining and suite of particle picking tools in 2D and 3D

  • High-resolution: advanced 3D refinement and classification tools achieve state-of-the-art results

  • Scalability: small storage footprint and distributed computing allows analysis of large datasets

  • On-the-fly processing: real-time analysis of micrographs and tilt-series during data acquisition

  • Portability and compatibility: easy to install and configure, interoperable with external packages

See the Changelog for the latest features.

Citing nextPYP

Liu, HF., Zhou, Y., Huang, Q., Piland, J., Jin, W., Mandel, J., Du, X., Martin, J., Bartesaghi, A., nextPYP: a comprehensive and scalable platform for characterizing protein variability in-situ using single-particle cryo-electron tomography. Nat Meth, 20:1909–1919 (2023).