Filter micrographs/tilt-series

Filters allow users to select a subset of micrographs or tilt-series to be processed downstream.

Creating filters

  • Click inside the Pre-processing block and go to the Table view.

  • Create a new filter by providing a name and clicking Save.

  • Add new criteria to the filter using the +Add button.

  • Select the desired parameter from the dropdown list and set the desired range using the sliders.

  • Use Apply filters to preview the results of the filter.

  • You can also manually include or exclude individual micrographs or tilt-series using the Exclude column in the table. Shortcuts are provided to facilitate the labeling of large datasets, for example, type x to exclude and c to include an image and advance to the next or previous entry in the table.

  • Once you are satisfied with the results, click Save to store the filter settings.

Here is a screenshot showing the use of filters in the Pre-processing block:

Create new filter

Tips

  • You can create multiple filters by providing a new name and clicking Save.

  • Previously saved filters can be retrieved by clicking Load and selecting the name of the filter from a list.

  • Once a filter is loaded, it can be edited, or deleted by clicking Delete (this operation cannot be undone).

Applying filters

  • Filters are applied to blocks downstream of the Pre-processing block

  • Create a new block downstream from the Pre-processing block. Select the name of the desired filter from the Filter micrographs or Filter tilt-series dropdown menu, and click Save. When you execute the new block, only the micrographs (or tilt-series) selected by the filter will be processed.

Select filter

Tips

  • Filters are only applied when first running blocks downstream of the Pre-processing block. If a filter is updated or a different filter is selected, the option to Delete files and data must be selected before re-running the block (or a new block downstream of the Pre-processing should be created).

  • You can experiment using different subsets of micrographs or tilt-series by creating multiple Particle refinement blocks and selecting a different filter for each block.