Filter micrographs/tilt-series¶

nextPYP allows users to select a subset of micrographs or tilt-series for further processing through the use of Filters.

Step 1: Create a filter¶

Click inside the Pre-processing block and go to the Table view.
Create a new filter by providing a name and clicking Save.
Add new criteria to the filter using the +Add button.
Select the desired parameter from the dropdown list and set the range using the sliders.
Use Apply filters to preview the results of the filter.
You can also manually include or exclude individual micrographs or tilt-series using the Exclude column in the table. Shortcuts are provided to facilitate the labeling of large datasets: type x to exclude and c to include an image and advance to the next or previous entry in the table.
Once you are satisfied with the results, click Save to save the filter settings.

Tip

You can create additional filters by providing a different name and clicking Save.
Saved filters can be retrieved by clicking Load and selecting the name of the filter from the list.
Once a filter is loaded, it can be edited or deleted by clicking Delete (this operation cannot be undone).

Create a new refinement block downstream from the Pre-processing block. Select the name of the desired filter from the Filter micrographs or Filter tilt-series dropdown menu, and click Save. When you execute the Particle refinement block, only the micrographs (or tilt-series) selected by the filter will be used for the processing downstream.

Tip

Filters do not work if you have previously executed the Particle refinement block without using a filter or using a different filter (you need to create a new Particle refinement block in this case).
You can experiment using different subsets of micrographs or tilt-series by creating multiple Particle refinement blocks and selecting a different filter for each block.