prismpyp metadata_cryosparc

Purpose

Convert micrograph .mrc files and power spectra .mrc files output by CTFFIND into .webp images, saving them to a new directory.

Additionally, this command parses the outputs of Patch CTF Estimation and CTFFIND4 to retrieve heuristic metrics, including:

• CTF fit
• Estimated resolution
• Mean defocus
• Relative ice thickness

Usage

usage: prismpyp metadata_cryosparc [-h] 
                                   [--patch-ctf-file PATCH_CTF_FILE] 
                                   [--ctffind-file CTFFIND_FILE] 
                                   [--ctffind-dir CTFFIND_DIR]
                                   [--imported-dir IMPORTED_DIR] 
                                   [--output-dir OUTPUT_DIR]

Named Arguments

Argument	Description
--patch-ctf-file	Path to cryoSPARC Manually Curate Exposures job exported .cs file
--ctffind-file	Path to cryoSPARC CTFFIND job exported .cs file
--ctffind-dir	Path to directory containing CTFFIND output .mrc files
--imported-dir	Path to directory containing imported micrographs
--output-dir	Output directory to save metadata and generated .webp images