nextPYP: a scalable platform for cryo-EM/ET image analysis
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`nextPYP <https://nextpyp.app/>`_ is a comprehensive platform for single-particle cryo-EM/ET image analysis developed and maintained by the `Bartesaghi Lab <http://cryoem.cs.duke.edu>`_ at `Duke University <http://www.duke.edu>`_.

Main features
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- Portable, easy-to-use, fully featured web-based GUI
- On-the-fly data pre-processing (single-particle and tomography)
- End-to-end pipeline for high-resolution structure determination (single-particle and tomography)
- 2D/3D particle picking using size-based, geometry-based and neural network-based approaches
- Scalable, small storage footprint (no need to save full size tomograms, sub-volumes, or particle stacks)
- Multi-mode constrained refinement and constrained classification for heterogeneity analysis
- Particle-based CTF refinement, movie frame refinement and self-tuning exposure weighting
- Import/export metadata in .star format to interface with external programs

Select from the following options:

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.. admonition:: Need help?

   Visit ``nextPYP``'s `discussion board <https://github.com/orgs/nextpyp/discussions>`_ to post questions and follow discussions

.. admonition:: Citing ``nextPYP``

  Liu, HF., Zhou, Y., Huang, Q., Piland, J., Jin, W., Mandel, J., Du, X., Martin, J., Bartesaghi, A., `nextPYP: a comprehensive and scalable platform for characterizing protein variability in-situ using single-particle cryo-electron tomography <https://www.nature.com/articles/s41592-023-02045-0>`_. Nature Methods, 20:1909–1919 (2023).